Volume 3, Issue 1-2, February 2015, Page: 111-114
Optimizing the Polysaccharide Extraction from the Vietnamese Lingzhi (Ganoderma lucidum) Via Enzymatic Method
Dam Sao Mai, Institute of Biotechnology and Food technology, Industrial University of Ho Chi Minh City, Ho Chi Minh City, Vietnam
Tu Thai Binh, Institute of Biotechnology and Food technology, Industrial University of Ho Chi Minh City, Ho Chi Minh City, Vietnam
Than Thi Ut Xi, Institute of Biotechnology and Food technology, Industrial University of Ho Chi Minh City, Ho Chi Minh City, Vietnam
Nguyen Thi Ngoc Tram, Natural University of Ho Chi Minh City, Ho Chi Minh City, Vietnam
Ngo Ke Suong, Dong Nai University of Technology, Bien Hoa City, Dong Nai province, Vietnam
Received: Oct. 5, 2014;       Accepted: Oct. 28, 2014;       Published: Jan. 29, 2015
DOI: 10.11648/j.jfns.s.2015030102.31      View  3977      Downloads  240
Abstract
Ganoderma lucidum is commonly used in Vietnam and other countries since more than 4000 years ago with excellent beneficial health effects Lingzhi has potential applications as a functional product with polysaccharide, and many other healthy components. This polysaccharide obtained good application in human. Lingzhi becomes a common mushroom in Vietnam, growing almost all year. The fruit of this mushroom contains high healthy polysaccharide. The polysaccharide extraction was surveyed and the enzymatic method of the extraction was optimized. The experiment was conducted according to a central composite design, with the following variables: Lingzhi-to-water ratio (L/W, 1:20–10:1), solvent temperature (ST, 35–55°C), enzyme concentration (EC, 0 – 0.8mL/250mL ~ 0 – 3.2%), and extraction time (ET, 1–7 hrs). Lingzhi were ground and mixed with the solvent, treated with enzyme, filtered and evaluated for polysaccharide contents. The polysaccharide extraction parameters were optimized. The result showed that the best adequate extraction conditions were L/W, 1:50; ST, 50°C; EC, 0.63mL/250mL ~ 0.25 %; and ET, 2.34 hrs. At such condition, 5.24% polysaccharide was extracted. The method is simple and can apply in the industry.
Keywords
Ganoderma lucidum, polysaccharide, Lingzhi, Enzyme, Optimizing Extraction
To cite this article
Dam Sao Mai, Tu Thai Binh, Than Thi Ut Xi, Nguyen Thi Ngoc Tram, Ngo Ke Suong, Optimizing the Polysaccharide Extraction from the Vietnamese Lingzhi (Ganoderma lucidum) Via Enzymatic Method, Journal of Food and Nutrition Sciences. Special Issue: Food Processing and Food Quality. Vol. 3, No. 1-2, 2015, pp. 111-114. doi: 10.11648/j.jfns.s.2015030102.31
Reference
[1]
Bao X, Liu C, Fang J, Li X. 2001. Structural and immunological studies of a major polysaccharide from spores of Ganoderma lucidum (Fr.) Karst. Carbohydr Res. 332:67–74.
[2]
Hikino H, Konno C, Mirin Y, Hayashi T. 1985. Isolation and hypoglycemic activity of ganoderans A and B, glycans of Ganoderma lucidum fruit bodies. Planata Med. 4:339–40
[3]
KV Harivaindaran, OPS Rebecca, S Chandran. 2008. Study of Optimal Temperature, pH and Stability of Dragon Fruit (Hylocereus polyrhizus). Pakistan Journal of Biological Sciences. Vol. 11: 2259-2263
[4]
Lee K. M, Lee S. Y, Lee H. Y. 1999. Bistage control of pH for improving exopolysaccharide production from mycelia of Ganoderma lucidum in an air-lift fermentor. J Biosci Bioeng. 88:646–50.
[5]
Miyazaki T, Nishijima M. 1981. Studies on fungal polysaccharides. XXVII. Structural examination of a water-soluble, antitumor polysaccharide of Ganoderma lucidum. Chem Pharm Bull. 29:3611–16
[6]
Nguyen Minh Tu. 2009. The process of bioactive extraction from Lingzhi. J. Scientist and Technology, Vietnam. Vol 47 (1)
[7]
Nguyen Tuan Anh, Tran Thu Huong, Nguyen Thi Phuong Ha. 2007. Survey on the conditions to extract the bioactive components from Lingzhi (Garnoderma lucidum Karst.). The proceeding of the 4th Organics Chemistry Conference of Science and Technology in Vietnam.
[8]
Tomoda M, Gonda R, Kasahara Y, Hikino H. 1986. Glycan structures of ganoderans B and C, hypoglycemic glycans of Ganoderma lucidum fruit bodies. Phytochemistry. 25:2817–20
[9]
Wachtel-Galor S, Buswell J. A, Tomlinson B, Benzie I. F. F. 2004. Lingzhi polyphorous fungus. In: Herbal and Traditional Medicine: Molecular Aspects of Health. New York: Marcel Dekker Inc. pp. 179–228.
[10]
Wang Y. Y, Khoo K. H, Chen S. T, Lin C. C, Wong C. H, Lin C. H. 2002. Studies on the immunomodulating and antitumor activities of Ganoderma lucidum (Reishi) polysaccharides: Functional and proteomic analyses of a fucose-containing glycoprotein fraction responsible for the activities. Bioorg Med Chem..10:1057–62.
Browse journals by subject