Volume 3, Issue 1-2, February 2015, Page: 28-32
Adhesive Microflora on Stainless Steel Coupons in Seafood Processing Plant
Bui Thi Quynh Hoa, Department of Food Science and Technology, Agro-Industry Faculty, Kasesart University, Bangkok 10900, Thailand
Warapa Mahakarnchanakul, Department of Food Science and Technology, Agro-Industry Faculty, Kasesart University, Bangkok 10900, Thailand
Tanaboon Sajjaanantakul, Department of Food Science and Technology, Agro-Industry Faculty, Kasesart University, Bangkok 10900, Thailand
Vichien Kitpreechavanich, Department of Microbiology, Faculty of Science, Kasesart University, Bangkok 10900, Thailand
Received: Oct. 5, 2014;       Accepted: Oct. 25, 2014;       Published: Jan. 29, 2015
DOI: 10.11648/j.jfns.s.2015030102.15      View  3430      Downloads  227
Adhering microflora profile is essential for the development and design of cleaning and sanitizing procedures, which is a part of the HACCP system. The profile provides a complete view of the target microorganisms that can potentially contaminate food products. The aim of this study is, therefore, focused on examination of microbial population adhered to the equipment surfaces at seafood processing plant. Adhering microflora was evaluated by installing the stainless steel coupons on the surface of equipment. Seventy stainless steel coupons were attached on 7 different locations at seafood processing plant and then fourteen coupons were collected at 1, 2, 3, 5 and 9 weeks interval. The population of bacteria adhered on samples were enumerated using swab test and spread plate method on different selective microbiological media. The total viable count and Pseudomonas spp. population found on the stainless steel coupons were ranged from 101 to 106cfu/cm2. The results also indicated that mature biofilm might form at some locations after three weeks. After 5 weeks, the highest viable bacterial population (106cfu/cm2) was observed on the stainless steel coupons attached in the trolley used for carrying the soup. These finding supported that the appropriate cleaning and sanitizing procedure should be strictly applied to assure safety in food processing plant.
Biofilm Formation, Microflora, Stainless Steel Coupon, Seafood Processing
To cite this article
Bui Thi Quynh Hoa, Warapa Mahakarnchanakul, Tanaboon Sajjaanantakul, Vichien Kitpreechavanich, Adhesive Microflora on Stainless Steel Coupons in Seafood Processing Plant, Journal of Food and Nutrition Sciences. Special Issue: Food Processing and Food Quality. Vol. 3, No. 1-2, 2015, pp. 28-32. doi: 10.11648/j.jfns.s.2015030102.15
Bagge-Ravn, D. (2003). The microbial ecology of processing equipment in different fish industries—analysis of the microflora during processing and following cleaning and disinfection. International Journal of Food Microbiology, 87(3), 239-250.
Bower, C. K., McGuire, J., & Daeschel, M. A. (1996). The adhesion and detachment of bacteria and spores on food-contact surfaces. Trends in Food Science & Technology, 7(152-157).
Chmielewski, R. A. N., & Frank, J. F. (2003). Biofilm Formation and Control in Food Processing Facilities. Comprehensive Reviews in Food Science and Food Safety, 2, 22-32.
Giaouris, E., Heir, E., Hebraud, M., Chorianopoulos, N., Langsrud, S., Moretro, T., Habimana, O., Desvaux, M., Renier, S., & Nychas, G. J. (2014). Attachment and biofilm formation by foodborne bacteria in meat processing environments: Causes, implications, role of bacterial interactions and control by alternative novel methods. Meat Sci., 97, 298-309.
Zottola, E. A., & Sasahara, K. C. (1994). Microbial biofilms in the food processing industry - Should they be a concern? International Journal of Food Microbiology, 23 125-148.
Jeong, D. K., & Frank, J. F. (1994). Growth of Listeria monocytogenes at 21oC in Biofilms with micro-organisms Isolated from Meat and Dairy Processing Environments. LWT-Food Science and Technology, 27(415-424).
Lavilla, L., L., Benomar, N., Galvez, A., & Abriouel, H. (2013). Prevalence of bacteria resistant to antibiotics and/or biocides on meat processing plant surfaces throughout meat chain production. International Journal of Food Microbiology, 161(2), 97-106.
Mah, T.-F. C., & O’Toole, G. A. (2001). Mechanisms of biofilm resistance to antimicrobial agents in Microbiology. Trends in Microbiology, 9(1), 34-39.
Branda, S. S., Vik, S., Friedman, L., & Kolter, R. (2005). Biofilms: the matrix revisited. Trends in Microbiology, 13(1), 20-26.
Donlan, R. M. (2002). Biofilms: Microbial Life on Surfaces. Emerging Infectious Diseases PERSPECTIVE, 8( 9), 881-890.
Giaouris, E., Chorianopoulos, N., Skandamis, P., & Nychas, G.-J. (2012). Attachment and Biofilm Formation by Salmonella in Food Processing Environments In B. S. M. Mahmoud (Ed.), Salmonella - A Dangerous Foodborne Pathogen (pp. 450). Croatia: InTech.
Khamisse, E., Firmesse, O., Christieans, S., Chassaing, D., & Carpentier, B. (2012). Impact of cleaning and disinfection on the non-culturable and culturable bacterial loads of food-contact surfaces at a beef processing plant. International Journal of Food Microbiology, 158(2), 163-168.
van der Veen, S., & Abee, T. (2011). Mixed species biofilms of Listeria monocytogenes and Lactobacillus plantarum show enhanced resistance to benzalkonium chloride and peracetic acid. International Journal of Food Microbiology, 144(3), 421-431.
Costerton, J. W., Stewart, P. S., & Greenberg, E. P. (1999). Bacterial Biofilms: A Common Cause of Persistent Infections. Science, 284(5418), 1318-1322.
Eileen, B. S., & Amy, C. L. W. (2004). Efficacy of Two Cleaning and Sanitizing Combinations on Listeria monocytogenes Biofilms Formed at Low Temperature on a Variety of Materials in the Presence of Ready-to-Eat Meat Residue. Journal of Food Protection, 67(10), 2218-2229.
Kumar, C. G., & Anand, S. K. (1998). Significance of microbial biofilms in food industry: a review. International Journal of Food Microbiology, 42, 9-27.
Arciola, C. R., Campoccia, D., Speziale, P., Montanaro, L., & Costerton, J. W. (2012). Biofilm formation in Staphylococcus implant infections. A review of molecular mechanisms and implications for biofilm-resistant materials. Biomaterials, 33(26), 5967-5982.
Pan, Y., Breidt, F. J., & Kathariou, S. (2006). Resistance of Listeria monocytogenes biofilms to sanitizing agents in a simulated food processing environment. Applied and Environmental Microbiology, 72(12), 7711-7717.
Poimenidou, S., Belessi, C. A., Giaouris, E. D., Gounadaki, A. S., Nychas, G. J., & Skandamis, P. N. (2009). Listeria monocytogenes attachment to and detachment from stainless steel surfaces in a simulated dairy processing environment. Applied and Environmental Microbiology, 75(22), 7182-7188.
Rodrigues, D. A. F. (2010). Listeria monocytogenes and Salmonella enterica adhesion, biofilm formation and control. Universidade do Minho, Escola de Engenharia.
Sauer, K. C., A. K. Ehrlich, G. D. Costerton, J. W. Davies, D. G. (2002). Pseudomonas aeruginosa Displays Multiple Phenotypes during Development as a Biofilm. Journal of Bacteriology, 184(4), 1140-1154.
Yang, L., Liu, Y., Wu, H., Hoiby, N., Molin, S., & Song, Z. J. (2011). Current understanding of multi-species biofilms. Int J Oral Sci, 3(2), 74-81.
Dourou, D., Beauchamp, C. S., Yoon, Y., Geornaras, I., Belk, K. E., Smith, G. C., Nychas, G. J., & Sofos, J. N. (2011). Attachment and biofilm formation by Escherichia coli O157:H7 at different temperatures, on various food-contact surfaces encountered in beef processing. International Journal of Food Microbiology, 149(3), 262-268.
Fuster-Valls, N., Hernández-Herrero, M., Marín-de-Mateo, M., & Rodríguez-Jerez, J. J. (2008). Effect of different environmental conditions on the bacteria survival on stainless steel surfaces. Food Control, 19(3), 308-314.
Helke, D., Somers, E., & Wong, A. (1993). Attachment of Listeria monocytogenes and Salmonella typhimurium to stainless steel and Buna-N in the presence of milk and individual milk components. Journal of food protection (USA).
Birna, G., Einarsson, H., & Thorkelsson, G. (2005). Microbial Adhesion to Processing Lines for Fish Fillets and Cooked Shrimp: Influence of Stainless Steel Surface Finish and Presence of Gram-Negative Bacteria on the Attachment of Listeria monocytogenes. Food Technology and Biotechnology, 43(1), 55-61.
Gunduz, G. T., & Tuncel, G. (2006). Biofilm formation in an ice cream plant. Antonie Van Leeuwenhoek, 89(3-4), 329-336.
Hood, S. K., & Zottola, E. A. (1997). Isolation and Identification of Adherent Gram-Negative Microorganisms from Four Meat-Processing Facilities. Journal of Food Protection, 60(9), 1135-1138.
Marchand, S., Block, D. J., Jonghe, D. V., Coorevits, A., Heyndrickx, M., & Lieve, H. (2012). Biofilm Formation in Milk Production and Processing Environments; Influence on Milk Quality and Safety. Comprehensive Reviews in Food Science and Food Safety, 11(2), 133-147.
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