Volume 8, Issue 1, January 2020, Page: 15-23
Estimation of Micronutrient Contents in Traditional Green Leafy Vegetables and Their Potential Contribution to Dietary Recommended Intakes
Faustina Dufie Wireko-Manu, UMR408 SQPOV «Sécurité et Qualité des Produits d'Origine Végétale», INRAE, Avignon Université, Avignon, France; Department of Food Science and Technology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana
Shirley Isibhakhomen Ejoh, UMR408 SQPOV «Sécurité et Qualité des Produits d'Origine Végétale», INRAE, Avignon Université, Avignon, France; Department of Human Nutrition, Faculty of Public Health, College of Medicine, University of Ibadan, Ibadan, Nigeria
David Page, UMR408 SQPOV «Sécurité et Qualité des Produits d'Origine Végétale», INRAE, Avignon Université, Avignon, France
Catherine M. G. C. Renard, UMR408 SQPOV «Sécurité et Qualité des Produits d'Origine Végétale», INRAE, Avignon Université, Avignon, France
Received: Feb. 21, 2020;       Accepted: Mar. 23, 2020;       Published: Apr. 7, 2020
DOI: 10.11648/j.jfns.20200801.13      View  113      Downloads  50
Micronutrient deficiency is a public health problem even though vegetable consumption could avert it. Vitamin C [Ascorbic acid (AA) and dehydroascorbate (DHAA)], β-carotene, lutein and vitamin B9 contents in seven traditional green leafy vegetables (raw and cooked) from Ghana were determined, to identify good sources and their potential contribution to Dietary Recommended Intakes. The micronutrients were quantified using spectrofluorimetric and HPLC/DAD analytical systems. Vitamin C content of samples ranged between 7.2 and 161 mg/100 g fresh weight. β-carotene content was within the ranges of 2.97 to 10.35 mg/100 g, Lutein 13.5 to 31.6 mg/100 g and total folate 18 to 146 μg /100 g. Lutein and β-carotene were in variable relative proportions (L/C from 1.6 to 6); Solanum macrocarpon and Amaranthus hybridus samples were particularly rich in lutein and β-carotene, respectively. Losses between 45 and 94% were observed for vitamin C, between 15 to 81% for β-carotene with the exception of an increase in Solanum macrocarpon and 17 to 80% for lutein under boiling. Similar drastic losses were recorded in microwaved samples; however, losses in lutein and folate were comparatively lower. The traditional green leafy vegetables studied were found to be very rich in the studied micronutrients, but cooking led to considerable losses. However, the cooked vegetables represent non-negligible sources of folate, good source of lutein and could provide up to 97% and 90% vitamin A and C Recommended Dietary Intakes respectively, when a 100 g is consumed. Improved cooking methods over the traditional methods of preparation are essential for retaining more micronutrients, for the benefit of consumers.
Traditional Green Leafy Vegetables (TGLV), Ascorbic Acid, β-Carotene, Lutein, Folate, Cooking Methods
To cite this article
Faustina Dufie Wireko-Manu, Shirley Isibhakhomen Ejoh, David Page, Catherine M. G. C. Renard, Estimation of Micronutrient Contents in Traditional Green Leafy Vegetables and Their Potential Contribution to Dietary Recommended Intakes, Journal of Food and Nutrition Sciences. Vol. 8, No. 1, 2020, pp. 15-23. doi: 10.11648/j.jfns.20200801.13
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Darnton-Hill, I., Webb, P., Harvey, P. W., Hunt, J. M., Dalmiya, N., Chopra, M., Ball, M. J. Bloem, M. W. and de Benoist, B. (2005) ‘Micronutrient deficiencies and gender: social and economic cost’, The American Journal of Clinical Nutrition, 81 (5), p. 1198S–1205S. doi: 81/5/1198S [pii].
Smith, F. I. and Eyzaguirre, P. (2007). African leafy vegetables; their role in the World Health Organaization’s global fruit and vegetables initiative. Available from http://tspace.library.utoronto.ca/handle/1807/55368.
Amo-Adjei J, Kumi-Kyereme A (2014) Fruit and vegetable consumption by ecological zone and socioeconomic status in Ghana. Journal of Biosocial Science 47 (05): 1–19.
Uusiku, N. P., Oelofse, A., Duodu, K. G., et al. (2010). Nutritional value of leafy vegetables of sub-Saharan Africa and their potential contribution to human health: a review. J. Food Comp Anal, 23 (6): 499-509.
Darkwa S, D A A (2013). The use of indigenous green leafy vegetables in the preparation of Ghanaian dishes. Journal of Food Processing and Technology, 04 (12).
Gupta, S., Jyothi, L. A, Manjunath, M. N., et al. (2005). Analysis of nutrient and antinutrient content of underutilized green leafy vegetables. LWT-Food Science and Technology, 38 (4): 339-45.
Delchier, N., Herbig, A. L., Rychlik, M. and Renard, C. M. G. C. (2016). Folates in Fruits and Vegetables: Contents, Processing, and Stability. Comprehensive Reviews in Food Science and Food Safety, 15: 506-528. doi: 10.1111/1541-4337.12193
Amusan, E. A., Ejoh, S. I., and Akinyele, I. O. (2015). Effect of boiling and steaming methods on the ascorbic acid content of wild and cultivated indigeneous green leafy vegetables found in Ibadan, Oyo State. Nigerian Journal of Nutrition Science, 36 (2): 59-63.
Bureau S, Mouhoubi S, Touloumet L, Garcia C, Moreau F, Bedouet V and Renard C M G C (2015). Are folates, carotenoids and vitamin C affected by cooking? Four Domestic procedures are compared on a large diversity of vegetables. LWT-Food Science and Technology, 64: 735-741.
Schonfeldt, H C. and Pretoriuos, B. (2011). The nutrient content of five traditional South African dark green leafy vegetables – A preliminary study. Journal of Food Composition and Analysis 28 (8): 1141-6.
Jaarsveld P V, Faber M, Heerden I V, Wenhold F, Jansen W, Rensburg V, Averbeke W V (2014). Nutrient content of eight African leafy vegetables and their potential contribution to dietary reference intakes. Journal of Food Composition and Analysis 33 (1): 77–84.
Duru, M., Tallowin, J. and Cruz, P. (2005). Functional diversity in low input grassland farming systems: characterization, effect and management. Agronomy Research 3: 125–138
Osonwa, U., Uronnachi, E., Umeyor, C. and Onwuzuligbo, C. (2012). Physicotechnical properties of paracetamol tablets formulated with the gum from the fresh leaves of corchorus olitorius. International Journal of Pharmaceutical Sciences and Research. 3. 2676-2679.
Oke, O. L. 1983. Amaranth. In "Handbook of Tropical Foods," ed. H. T. Chan Jr., p. 1-2. Marcel-Dekker, Inc., New York.
Mepha, H. D., Eboh, L. and Banigbo, D. E. B. (2007). Effect of processing treatments on the nutritive composition and consumer acceptance of some Nigerian edible leafy vegetables. African Journal of Food Agricultural Nutrition Development, 7 (1): 1–18.
Duru, M., Amadi, C., Ugbogu, A., Eze, A. and Amadi, B. (2012). Phytochemical, vitamin and proximate composition of Dacryodes edulis fruit at different stages of maturation. Asian Journal of Plant Science and Research 2 (4): 437-441.
Stevens R, Buret M, Garchery C, Yolande Carretero A, Causse† M (2006). Technique for rapid, small-scale analysis of vitamin C levels in fruit and application to a tomato mutant collection. Journal of Agricultural and Food Chemistry 54 (17): 6159–6165.
Delchier, N., Ringling, C., Le Grandois, J., Aoudé-Werner, D., Galland, R., Georgé S., Rychlik, M. and Renard, C. M. (2013). Effects of industrial processing on folate content in green vegetables. Food Chemistry, 139 (1-4): 815-24. doi: 10.1016/j.foodchem.2013.01.067.
Ogunlesi M, Okiei W, Azeez L, Obakachi V, Osunsanmi M, Nkenchor G (2010). Vitamin C contents of tropical vegetables and foods determined by voltammetric and titrimetric methods and their relevance to the medicinal uses of the plants. International Journal of Electrochemical Science, 5: 105–115.
Chang S K, Nagendra P K, Amin I (2013). Carotenoids retention in leafy vegetables based on cooking methods. International Food Research Journal, 20 (1): 457-465.
Raju M, Varakumar S, Lakshminarayana R, Krishnakantha T P, Baskaran V (2007). Carotenoid composition and vitamin A activity of medicinally important green leafy vegetables. Food Chemistry 101 (4): 1598–1605.
Agbemafle R, Obodai EA, Adukpo GE, Amprako DN (2012) Effects of boiling time on the concentrations of vitamin c and beta-carotene in five selected green vegetables consumed in Ghana. Advances in Applied Science Research, 3 (5): 2815–2820.
Lee S K, Kader A A (2000). Preharvest and postharvest factors influencing vitamin C content of horticultural crops. Postharvest Biology and Technology 20 (3): 207–220.
Ellong, E. N., Billard, C., Adenet, S., Rochefort, K. (2015). Polyphenols, Carotenoids, Vitamin C Content in Tropical fruits and vegetable and impact of processing methods. Journal of Food and Nutrition Sciences. p. 299-313. doi: 10.4236/fns.2015.63030.
Steiner-Asiedu M, Agbemafle I, Setorglo J, Danquah A O, Anderson A K (2014). Carotenoids content of Corchorus olitorius and Solanum macrocarpon - commonly used Ghanaian vegetables. International Food Research Journal, 21 (5): 2063–2067.
Pakistan P, Nauman Ahamad M, Saleemullah M, Ullah Shah H, Khalil I A, Saljoqi A (2007). Determination ofcarotene content in raw vegetatbles using high performance liquid chromatography. Sarhad Journal of Agriculture 23 (3): 767–770.
FAO / IN FOODS. (2012). In Guidelines Guidelines for Converting Units, Denominators and Expressions (p. A–6. (2010a), 2010. A–6. (2010b), 2010. A–6. (2010).
Djuikwo V N, Ejoh R A, Gouado I, Mbofung C M and Tanumihardjo S A (2011). Determination of major carotenoids in processed tropical leafy vegetables indigenous to Africa. Food and Nutrition Sciences 2: 793–802.
SanGiovanni J P, Chew E Y, Clemons T E, et al. (2007). The relationship of dietary lipid intake and age-related macular degeneration: A case-control study in the Age-Related Eye Disease Study. Archives of Ophthalmology, 125 (5): 671–679.
Delchier N, Reich M, Renard C M G C (2012). Impact of cooking methods on folates, ascorbic acid and lutein in green beans (Phaseolus vulgaris) and spinach (Spinacea oleracea). LWT - Food Science and Technology 49 (2): 197–201.
Konings E J M, Roomans H H S, Dorant E, Goldbohm R A, Saris W H M, van den Brandt P A (2001). Folate intake of the Dutch population according to newly established liquid chromatog- raphy data for foods. American Journal of Clinical Nutrition, 73 (4): 765–76.
Ejoh, S. I., Wireko-Manu, F. D., Page, D. and Renard, C. M. G. C. (2019). Traditional green leafy vegetables as underutilized sources of micronutrients in a rural farming community in South-west Nigeria I: estimation of vitamin C, carotenoids and mineral contents. South African Journal of Clinical Nutrition, pp. 1-6. DOI: 10.1080/16070658.2019.1652963.
Ogle, B., Dao, H., Generose, M and Leif, H. (2001). Micronutrient composition and nutritional importance of gathered vegetables in Vietnam. International Journal of Food Sciences and Nutrition, 52: 485-99. DOI: 10.1080/713671806.
Shohag M J I, Yanyan W, Ning Y, Lingli L, Jie Z, Zhenli H, Johan P, Xiaoe Y (2012). Folate Content and Composition of Vegetables Commonly Consumed in China. Journal of Food Science 77 (11): 239-245.
World Health Organization and Food and Agriculture Organization of the United Nations (2004). Vitamin and mineral requirements in human nutrition: report of a joint FAO/WHO expert consultation, Bangkok, Thailand
Okpalamma F, Ojimelukwe P C, Mazi E A (2013). Postharvest storage and processing changes in carotenoids and micronutrients in fluted pumpkin (Telferia occidentalis Hook F.). IOSR Journal of Agriculture and Veterinary Science 6 (4): 34–39.
Liu Y, Perera C O, Suresh V (2007). Comparison of three chosen vegetables with others from South East Asia for their lutein and zeaxanthin content. Food Chemistry 101 (4): 1533–1539.
Rodriguez-Amaya D B (1997). Carotenoids and Food Preparation: The Retention of Provitamin A Carotenoids in Prepared, Processed, and Stored Foods, OMNI/USAID, Washington.
Granado F, Olmedilla B, Blanco I, Rojas-Hidalgo, E (1992). Carotenoid composition in raw and cooked Spanish vegetables. Journal of Agricultural and Food Chemistry, 40 16): 2135–2140.
McKillop D J, Pentieva K, Daly D, Mcpartlin J M, Hughes J, Strain, J J, Scott J M, Mcnulty, H. (2002). The effect of different cooking methods on folate retention in various foods that are amongst the major contributors to folate intake in the UK diet. British Journal of Nutrition, 88: 681–688.
Dang J (2000). Folate retention in selected processed legumes. Food Chemistry 68 (3): 295–298.
Mertz C, Brat P, Caris-Veyrat C, Gunata Z (2010). Characterization and thermal stability of carotenoids and vitamin C of tamarillo fruit (Solanum betaceum Cav.). Food Chemistry 119 (2): 653–659.
Klein B P (1989). Retention of nutrients in microwave-cooked foods. Boletín de La Asociación Médica de Puerto Rico 81 (7): 277–9. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/2775405.
López-Berenguer C, Carvajal M, Moreno D A, García-Viguera C (2007). Effects of microwave cooking conditions on bioactive compounds present in broccoli inflorescences. Journal of Agricultural and Food Chemistry 55 (24): 10001–10007.
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