Volume 7, Issue 6, November 2019, Page: 79-86
Resistant Protein Promotes Glucagon-like Peptide-1 Secretion by Controlling the Cecal Fermentation in Rats Fed High Amylose Corn Starch
Ryoko Shimada, School of Human Science and Environment, University of Hyogo, Himeji, Japan; Faculty of Health Sciences, Osaka Aoyama University, Minoh, Japan
Junichi Matsumoto, Marutomo Co. Ltd., Iyo, Japan
Mikiharu Doi, Marutomo Co. Ltd., Iyo, Japan
Kiyoshi Ebihara, Faculty of Health Sciences, Osaka Aoyama University, Minoh, Japan
Received: Oct. 8, 2019;       Accepted: Oct. 25, 2019;       Published: Oct. 31, 2019
DOI: 10.11648/j.jfns.20190706.11      View  17      Downloads  14
Abstract
It was examined whether undigested protein, namely resistant protein, affects glucagon-like peptide-1 (GLP-1) secretion in rats fed high amylose corn starch (HACS). Rats were fed one of three experimental diets for 28 d: casein without HACS, casein with HACS or dashigara with HACS. Dashigara is produced from Katsuobushi (smoke-dried skipjack tuna) treated with microbial protease. The apparent digestibilities of casein and dashigara are 96.0% and 84.5%, respectively. The amount of rat cecal butyric acid and propionic acid were as follows: the dashigara with HACS group > the casein with HACS group > the casein without HACS group. The dashigara with HACS group had significantly greater cecal butyric acid and lower cecal succinic acid than the casein with HACS group. The GLP-1 concentration in portal vein blood increased as the amount of butyric acid and propionic acid in the cecal contents increased. Denaturing gradient gel electrophoresis of PCR-amplified 16S rRNA gene fragments showed that the composition of cecal microbiota differed among the three groups. These results suggest that resistant protein contained in dashigara promotes GLP-1 secretion by controlling fermentation efficiency as well as the fermentation profile of HACS through the changes in cecal microbiota in rats fed HACS.
Keywords
Glucagon-Like Peptide-1, High Amylose Corn Starch, Resistant Protein, Short Chain Fatty Acids, Cecal Microbiota, Cecal Fermentation
To cite this article
Ryoko Shimada, Junichi Matsumoto, Mikiharu Doi, Kiyoshi Ebihara, Resistant Protein Promotes Glucagon-like Peptide-1 Secretion by Controlling the Cecal Fermentation in Rats Fed High Amylose Corn Starch, Journal of Food and Nutrition Sciences. Vol. 7, No. 6, 2019, pp. 79-86. doi: 10.11648/j.jfns.20190706.11
Copyright
Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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